Scientific Production Directory
Articles
33861
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827
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7005
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23385
Recent Additions
  • Publication
    Phase 1 trial of malaria transmission blocking vaccine candidates Pfs25 and Pvs 25 formulated with montanide ISA 51
    ( 2008-07-09)
    Wu Y.
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    Ellis R.D.
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    Shaffer D.
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    Fontes E.
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    Malkin E.M.
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    Fay M.P.
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    Narum D.
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    Rausch K.
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    Miles A.P.
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    Aebig J.
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    Orcutt A.
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    Muratova O.
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    Song G.
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    Lambert L.
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    Zhu D.
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    Miura K.
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    Long C.
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    Saul A.
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    Miller L.H.
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    Durbin A.P.
    Background: Pfs25 and Pvs25, surface proteins of mosquito stage of the malaria parasites P. falciparum and P. vivax, respectively, are leading candidates for vaccines preventing malaria transmission by mosquitoes. This single blinded, dose escalating, controlled Phase 1 study assessed the safety and immunogenicity of recombinant Pfs25 and Pvs25 formulated with Montanide ISA 51, a water-in-oil emulsion. Methodology/Principal Findings: The trial was conducted at The Johns Hopkins Center for Immunization Research, Washington DC, USA, between May 16, 2005-April 30, 2007. The trial was designed to enroll 72 healthy male and non-pregnant female volunteers into 1 group to receive adjuvant control and 6 groups to receive escalating doses of the vaccines. Due to unexpected reactogenicity, the vaccination was halted and only 36 volunteers were enrolled into 4 groups: 3 groups of 10 volunteers each were immunized with 5 μg of Pfs25/ISA 51, 5 μg of Pvs25/ISA 51, or 20 μg of Pvs25/ISA 51, respectively. A fourth group of 6 volunteers received adjuvant control (PBS/ISA 51). Frequent local reactogenicity was observed. Systemic adverse events included two cases of erythema nodosum considered to be probably related to the combination of the antigen and the adjuvant. Significant antibody responses were detected in volunteers who completed the lowest scheduled doses of Pfs25/ISA 51. Serum anti-Pfs25 levels correlated with transmission blocking activity. Conclusion/Significance: It is feasible to induce transmission blocking immunity in humans using the Pfs25/ISA 51 vaccine, but these vaccines are unexpectedly reactogenic for further development. This is the first report that the formulation is associated with systemic adverse events including erythema nodosum.
  • Publication
    Combining Tabu search and genetic algorithms to solve the capacitated multicommodity network flow problem
    (National Institute for R and D in Informatics, 2014-01-01)
    Lagos C.
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    Crawford B.
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    Cabrera E.
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    Rubio J.
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    Paredes F.
    Network design has been an important issue in logistics during the last century. This is due to the significant impact that an efficient distribution network design can have over both costs and service level. In this article, we present a heuristic solution approach for the well-known capacitated multicommodity network flow problem. The heuristic approach combines two well-known algorithms namely Tabu Search and Genetic Algorithms. While the main algorithm is Tabu Search, the Genetic Algorithm is used to select the best option among the neighbours of the current solution. To be able to do that some well-known evolutionary operators such as cross-over and mutation are made use of. This hybrid approach obtains important improvements when compared to the ones presented previously in the literature.
  • Publication
    Patent filarial infection modulates malaria-specific type 1 cytokine responses in an IL-10-dependent manner in a filaria/malaria-coinfected population
    ( 2009-07-15)
    Metenou S.
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    Dembéle B.
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    Konate S.
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    Dolo H.
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    Coulibaly S.Y.
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    Coulibaly Y.I.
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    Diallo A.A.
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    Soumaoro L.
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    Coulibaly M.E.
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    Sanogo D.
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    Doumbia S.S.
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    Wagner M.
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    Traoré S.F.
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    Klion A.
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    ;
    Nutman T.B.
    The effect of filarial infections on malaria-specific immune responses was investigated in Malian villages coendemic for filariasis (Fil) and malaria. Cytokines were measured from plasma and Ag-stimulated whole blood from individuals with Wuchereria bancrofti and/or Mansonella perstans infections (Fil+; n = 19) and those without evidence of filarial infection (Fil-; n = 19). Plasma levels of IL-10 (geometric mean [GM], 22.8 vs 10.4) were higher in Fil+ compared with Fil-, whereas levels of IFN-inducible protein (IP)-10 were lower in Fil+ (GM, 66.3 vs 110.0). Fil+ had higher levels of spontaneously secreted IL-10 (GM, 59.3 vs 6.8 pg/ml) and lower levels of IL-2 (1.0 vs 1.2 pg/ml) than did Fil-. Although there were no differences in levels of Staphylococcus aureus enterotoxin B-induced cytokines between the two groups, Fil+ mounted lower IL-12p70 (GM, 1.11 vs 3.83 pg/ml; p = 0.007), IFN-γ (GM, 5.44 vs 23.41 pg/ml; p = 0.009), and IP-10 (GM, 29.43 vs 281.7 pg/ml; p = 0.007) responses following malaria Ag (MalAg) stimulation compared with Fil -. In contrast, Fil+ individuals had a higher MalAg-specific IL-10 response (GM, 7318 pg/ml vs 3029 pg/ml; p = 0.006) compared with those without filarial infection. Neutralizing Ab to IL-10 (but not to TGFβ) reversed the down-regulated MalAg-specific IFN-γ and IP-10 (p < 0.001) responses in Fil+. Together, these data demonstrate that filarial infections modulate the Plasmodium falciparum-specific IL-12p70/IFN-γ secretion pathways known to play a key role in resistance to malaria and that they do so in an IL-10-dependent manner.
  • Publication
    Agronomic, productive, nutritional and economic potential of four forage maize genotypes
    (Corporacion Colombiana de Investigacion Agropecuaria Corpoica, 2021-09-01)
    Maguiña-Maza R.M.
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    Perez S.C.F.
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    Cárdenas G.L.P.
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    Ameri N.H.C.
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    Abad H.N.P.
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    The objective of the research was to compare the agronomic, productive, nutritional and economic characteristics of four forage maize genotypes: INIA 617 Chuska, M28T Marginal 28 Tropical, Atlas 777 and INIA 619 Megahybrid, in the Chancay Valley - Peru. The four genotypes, with four replications each, were randomly distributed in 16 experimental units. Each experimental unit was represented by 16 rows of 29 m in length, spaced at 0.85 m, with a sowing distance between hits of 0.30 m and three seeds per hit. The agronomic characteristics were evaluated at 93 days of planting, while the productive, nutritional and economic yield were estimated at 110 days after planting. The INIA 617 and M28 genotypes reached the highest plant height, while INIA 619 and Atlas 777 showed the highest number of leaves and number of ears per plant, respectively. The maturity state factor of the forage only influenced the yield of neutral detergent fiber (NDF) and acid detergent fiber (ADF) and was observed in INIA 617 and M28T, genotypes harvested at the recommended maturity state. By effect of genotypes, Atlas 777 achieved the highest yield in green matter and ear weight, as well as the highest yield in protein, fat and ash, superiority possibly due to its stay green characteristic. Based on dry matter yield and utility, the four genotypes show similar productive and economic potential.
  • Publication
    Assessing effects of the e-Chasqui laboratory information system on accuracy and timeliness of bacteriology results in the Peruvian tuberculosis program.
    We created a web-based laboratory information system, e-Chasqui to connect public laboratories to health centers to improve communication and analysis. After one year, we performed a pre and post assessment of communication delays and found that e-Chasqui maintained the average delay but eliminated delays of over 60 days. Adding digital verification maintained the average delay, but should increase accuracy. We are currently performing a randomized evaluation of the impacts of e-Chasqui.